Topic outline


    Lecturer: Dr Sarni Mat Junit


    Creative Commons License
    MBBS Nucleid Acid Biochemistry: DNA and RNA by Sarni Mat Junit is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

  • DNA and RNA

    1. Introduction:

    •   Nucleic acid as the material that carries genetic information
    •   Direction of information flow is DNA – RNA – protein

    2. Nucleic acid as a polynucleotide:

    •   Nucleotides as basic building blocks
    •   Sugar structure - ribose and deoxyribose
    •   Purine and pyrimidine base structures
    •   Differences between ribonucleotides and deoxyribonucleotides
    •   NMP, NDP, NTP

    3. DNA structure:

    •   Conventional writing of nucleic acid structure.  5’ and 3’ end   polarity.
    •   Characteristics of Watson-Crick DNA model
    •   DNA denaturation, renaturation and Tm 
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